Surface Plasmon Resonance Assay for Label?Free and Selective Detection of <i>Xylella Fastidiosa</i>

Xylella fastidiosa is among the most dangerous plant bacteria worldwide causing a variety of diseases, with huge economic impact on agriculture and environment. A surveillance tool, ensuring highest possible sensitivity enabling early detection X. outbreaks, would be paramount importance. So far, pathogen biomarkers are studied by means surface plasmon resonance (SPR). Herein, multiparameter SPR (MP-SPR) used for first time to develop reliable label-free method fastidiosa. The real-time monitoring bioaffinity reactions provided as well. Selectivity guaranteed biofunctionalizing gold transducing interface polyclonal antibodies it assessed negative control experiment involving nonbinding Paraburkholderia phytofirmans bacterium strain PsJN. Limit 105 CFU mL?1 achieved direct interaction between its affinity antibody. Moreover, binding also evaluated, returning an constant 3.5 × 107 m?1, comparable those given in literature against antibodies.